Scientist - Clinical Research jobs in San Diego, CA

• Design, optimize, and characterize vector delivery platforms for in vivo gene editing of primary human immune cells.
• Generate retroviral, lenti, adeno, or adeno-associated vectors for the transduction of both established cell lines and primary immune cells.
• Apply state-of-the-art gene editing technologies, such as CRISPR, TALEN, and other endonucleases, and delivery approaches including multiple viral vector platforms and protein and mRNA electroporation.
• Perform cell biology, including isolation, characterization, and assay set up, with primary human and murine immune cells, tumor cell lines used in immuno-oncology research, and primary patient-derived cells.
• Design and execute project plans involving immune cell profiling, cell processing from xenograft models, cytotoxicity, proliferation, activation, cell viability, and survival assays with a strong focus on flow cytometry.
• Perform molecular biology experiments including DNA and protein preparation from cells, immunoprecipitation, western blots, etc.
• Prepare reports and present results at regular team meetings.

• A strong background in viral vector design and optimization of transduction protocols for gene therapies is essential.
• Experience with gene editing, cell transfection and transduction systems is essential.
• Experience with molecular biology techniques is essential.
• Hands-on experience with in vitroassays (co-culture assays) commonly used in immuno-oncology is preferred.
• A strong background in cell culture and multi-color flow cytometry is preferred.
• Experience and knowledge of the immune system is preferred. (Experience in T/B/NK/macrophage cell biology, tumor immunology).
• Hands-on experience with cellular immunotherapies is preferred but not required.
• Experience preparing reagents for in vivo assays and in vivo experience preferred but not required.
• Experience with protein engineering and antibody design is important.
• Experience using Prism, FlowJo, Office, and JMP software is important.
• Details of established essential functions for this position will be addressed/discussed during the interview process.

• BS in biology, chemical biology, biochemistry, or related field required. PhD is highly preferred.
• Minimum 2-5 years experience in industry is preferred, but academic candidates with strong skills matching the qualifications above will be considered as well.
• Impeccable attention to detail, and excellent ability to perform hands-on experiments, research, design, and organize project designs and maintain detailed documentation.
• Highly motivated, creative, and devoted to project goals.
• Able to collaborate proficiently and communicate effectively with colleagues and management teams.
• Able to contribute in a meaningful way to scientific discourse.

• Employer Contributed Retirement Plan Depending on eligibility, employees receive an employer contributed retirement plan (no employee contribution required) and the option to contribute to a 403(b) (which is similar to a 401(k) using your own pre or post-tax dollars)
• Full Suite of Health and Welfare plans including three medical plan options (including an HSA available option), dental, vision, life insurance, disability, EAP and more
• Access to Flexible Spending Accounts (Medical/Dependent Care)
• Competitive vacation and sick leave policies
• Free, on-site parking

About the Company:
RESEARCH SCIENTIST, Gene Therapy